The Diagnosis of Mitochondrial Diseases

Front cover: Mitochondrial morphology in human fibroblasts (cover micrographs). The panel at top left is a wild-type cell. Mitochondria (red structures) are labeled with MitoTracker Red TM ; tubulin, labeled in green, helps indicate overall cell structure. The panel at top right, labeled in a similar manner, shows a fibroblast from a patient with a mitochondrial disease. Note the appearance of many mitochondria as punctate structures, compared to the more tubular mitochondria in the wild-type cell. The panel at lower left shows mitochondria (stained with MitoTracker Red) co-localizing with a mitochondrial-associated protein (stained green with a fluorescently labeled antibody); the regions of red-green co-localization appear yellow. The panel at lower right is stained in the same way but, in addition, the endoplasmic reticulum is also stained, in blue. Clinical consultation and diagnosis Full range of enzyme diagnostics Analysis of mitochondrial DNA Research on mitochondria and their roles in diseaese Physician education Please see inside back cover for contact information.